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KMID : 1098420050130030069
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 3 p.69 ~ p.76
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Investigation of Medicinal Substances from in vitro Cultured Cells and Leaves of Artemisia princeps var. Orientalis
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Shin Dong-Ho
In Jun-Gyo
Yu Sang-Ryul
Choi Kwan-Sam
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Abstract
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The young leaves of A. princeps have been a well known for a crude medicine and used in treatment of colic pain, vomiting and menstrual irregularity. Based on TLC and HPLC and used an artemisinin, an anti-malarial compounds which is believed to be detected only in A. annuaup so far can be biosynthesized in A. princeps. To investigate the production of secondary metabolites like artemisinin in cultured cells, the cell culture of A. princeps was established. Callus and suspension cultured cells of A. princeps were induced and grown highest in MS media containing 0.2;mg/{ell} 2,4-D, 0.1;mg/{ell} BAP and 2% sucrose. Different metabolites from in vitro cultured cells (callus and suspension cultured cell) and intact plants were analyzed by TLC analysis. As a result, we can confirm that in vitro culture has a potential for mass production of secondary metabolites from A. princeps
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KEYWORD
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Artemisia princeps, artemisinin, medical compound, tissue cultured cells, TLC
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